Rb1lox PCR -- to detect recombination

Reaction Mix:

Tail DNA 100ng 1.0 ul
D1 (20uM) 0.5 ul
1lox 5' (20uM) 0.5 ul
3' lox (20 uM) 1 ul
milliQ 2 ul
Go Taq (Promega) 5 ul

PCR Primers:

D1 5' GCA GGA GGC AAA AAT CCA CAT AAC 3'
1lox 5' 5’ CTC TAG ATC CTC TCA TTC TTC CC 3’
3' lox 5’ CCT TGA CCA TAG CCC AGC AC 3’

PCR Conditions:

1. 94C 3:00
2. 94C 0:30
3. 55C 1:00
4. 72C 1:30
5. Goto 2 x30 cycles
6. 72C 5:00
END

Expected Bands:

Wt
250 bp
FLOX
310 bp
1 lox ~ 500bp