Reverse Complement DNA Sequence pAPCc-loxfNeof.seq(1,13288)
Created: Monday, July 12, 2004 12:05 AM

how this was made:

- pR26.1 was digested with NotI and SalI and the 5'-homology arm of APC's C-term was inserted between these sites to make pR26.1-APCc5

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ttcatctggagctaacaaaaatcaagtagacactaagaaaaagaagcctacttcaccagtaaagcccatgccacaaaatactgaatatagaacgcgtgtgagaaagaatacagactcaaaagttaatgtaaatactgaagaaactttctcagacaacaaagactcaaagaaaccaagcttacaaaccaatgccaaggccttcaatgaaaagctacctaacaatgaagacagagtgcgggggagcttcgccttggactcaccgcatcactacacccctattgaggggacgccgtactgcttttcccgaaatgactccttgagttctctggattttgatgatgacgatgttgacctttccagggaaaaggccgagttaagaaagggcaaagaaagcaaggattccgaagccaaagttacctgccgcccagaaccaaactcaagccagcaggcagctagtaagtcacaagccagtataaaacatccagcaaacagagcacagtccaaaccagtgctgcagaaacagcccactttcccccagtcctccaaagacggaccagatagaggggcagcaactgacgaaaaactgcagaattttgctattgaaaatactccagtttgcttttctcgaaattcctctctgagttcccttagtgacattgaccaggaaaacaacaataacaaagaaagtgaaccaatcaaagaagctgaacctgccaactcacaaggagagcccagtaagcctcaggcatccgggtatgctcccaagtccttccacgtcgaagacacccctgtctgtttctcaagaaacagctctctcagttctcttagcattgactctgaggacgacctgttacaggagtgtataagttctgccatgccaaaaaagaaaaggccttcaagactcaagagtgagagcgaaaagcagagccctagaaaagtgggtggcatattagctgaagacctgacgcttgatttgaaagatctacagaggccagattcagaacacgctttctcccccgactcagaaaattttgactggaaagctattcaggaaggcgcaaactccatagtaagtagtttgcaccaagctgctgcagccgccgcgtgcttatctagacaagcgtcatccgactcagattccattctgtcactaaagtccggcatttctctgggatcgccttttcatcttacacctgatcaagaggaaaagccattcacaagcaataaaggcccaagaattctcaaacctggagagaaaagcacattagaagcaaaaaaaatagaatctgaaaacaaaggaatcaaaggcgggaaaaaggtttataaaagcttgattacgggaaagattcgctccaattcagaaatttccagccaaatgaaacaacccctcccgacaaacatgccttcaatctcaagaggcaggacgatgattcacatcccagggcttcggaatagctcctctagtacaagccctgtctctaagaaaggcccacccctcaagactccagcctctaaaagccccagtgaagggccgggagctaccacttctcctcgaggaactaagccagcaggaaagtcagagcttagccctatcaccaggcaaacttcccaaatcagtgggtcaaataaggggtcttctagatcaggatctagagactccactccctcaagacctacacagcaaccattaagtaggccaatgcagtctccagggcgaaactcaatttcccctggtagaaatggaataagccctcctaacaaactgtctcagctgcccagaacatcatctcccagtactgcttcaactaagtcctccggttctgggaaaatgtcatatacatccccaggtagacagctgagccaacaaaatcttaccaaacaagcaagtttatccaagaatgccagcagtatccccagaagtgagtcggcatctaaaggactgaatcagatgagtaacggcaatgggtcaaataaaaaggtagaactttctagaatgtcttcaactaaatcaagtggaagtgaatcagacagatcagaaaggcctgcattagtacgccagtctactttcatcaaagaagccccaagcccaaccctgaggaggaaactggaggaatctgcctcatttgaatccctttctccatcttctagaccagattctcccaccaggtcgcaggcacagaccccagttttaagcccttcccttcctgatatgtctctgtccacacatccatctgttcaggcaggtgggtggcgaaagctcccgcctaatctcagccccactatcgagtataatgacggaaggcccacaaaacggcatgatattgcacgctcccattctgaaagtccttccagactaccaatcaaccgggcgggaacctggaagcgtgaacacagcaaacattcctcgtcccttcctcgagtgagtacttggagaagaactggaagctcatcttctattctttctgcttcatcagagtccagtgaaaaagcaaaaagtgaggatgaaaggcatgtgagctccatgccagcacccagacagatgaaggaaaaccaggtgcccaccaaaggaacatggaggaaaatcaaggaaagtgacatttctcccacaggcatggcttctcagagcgcttcctcaggtgctgccagtggtgctgaatccaagcctctgatctatcagatggcacctcctgtctctaaaacagaggatgtttgggtgagaattgaggactgccccattaacaaccctagatctggacggtcccccacaggcaacacccccccagtgattgacagtgtttcagagaagggaagttcaagcattaaagattcaaaagacacccatgggaaacagagtgtgggcagtggcagtcctgtgcaaaccgtgggtctggaaacccgcctcaactcctttgttcaggtagaggccccagaacagaaaggaactgaggcaaaaccaggacagagtaacccagtctctatagcagagactgctgagacgtgtatagcagagcgtacccctttcagttccagtagctccagcaagcacagctcacctagcgggactgttgctgccagagtgacaccttttaattacaaccctagccctaggaagagcagcgcagacagcacttcagcccggccgtctcagatccctacgccagtgagcaccaacacgaagaagagagattcgaagactgacagcacagaatccagtggagcccaaagtcctaaacgccattccgggtcttacctcgtgacgtctgtttaaaagaGCGGCCGCtctagaactagtggatccacctaataacttcgtatagcatacattatacgaagttatattatgtacctgactgatgaagttcctatactttctagagaataggaacttcggaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccGGTACCtggtgttaatcactactgctgtatagagactttatttcaaagaaaactttaaaggttgaaaatttttgtaaataggtttgattcttgttagaggggttttgttctggaaaccatatttgatagtacactttgtcctcactggtcattttgggaggcactcttattaggtaggaaaaaatggtaaagccaagtatatttgtacagtgtgttttacatgtatttaagtagcatccatcccattgtcctttaattatcacctgtcttaaaaataacactacagatagaaactatatgatatattgctgtcatcaatcacttttagattataaattaaacttacatcagggagaaattggtatttatgcaaaaaaaattagtccttgtgaatccatctaatgtcataattaatcatgtggctgtgaaattcacagtaatgtggttccccatgaatatgtttacccagcctgctttgctttactgcatgaatgaagctgacggttcaatttcagaagtaacaattaatgattctgtgatcacatggtgtgcagagagatagctacagtgtaacgattcacactattttgtgctcaaatctgtgtaactgtaaagcattgaatgaaactattttaactgaactagattttatcaaaagtaggtagaatttttgctatgctgtaactgttgtatattctggtatttgaggtgagttggctgctcttttattaatgagacacgaattgtgtctcaaactaaatgaacatttcagaataaattattgctgtatgtaaactattactgaaattggtatttgtttgagggtcttatttcacatttgtattaataactgtcaaaagggcctcttttaaaagctaaatgtaattttttttcttcagattctatgcattaagagtaaaatttcctcttgctgtaataaagacaattgaagaagattgttgacacttaaccattcc