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Jacks Lab

Koch Institute for Integrative Cancer Research at MIT

Massachusetts Institute of Technology

The Jacks Lab

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  • Jacks Lab Home
  • People
  • Research
    • Engineering hypermutant cancer models
    • Modeling cancer using CRISPR/Cas9 system
    • Epigenetic changes during lung cancer progression
    • Anti-oxidant response in lung tumorigenesis
    • Pancreatic cancer
    • Small cell lung cancer
    • Tumor evolution and homeostasis
    • Tumor microenvironment
    • Tumor immunology
  • Publications
    • Lab papers
    • Chapters and reviews
  • Protocols
  • Reagents
    • Addgene
    • MMHCC Mouse Repository
    • Jackson Laboratories
  • News & Events
    • News
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    • More lab events
  • Contact

Protocols

  • Adenovirus Cre Intranasal Infection
  • DNA Isolation - Proteinase K Method
  • DNA Isolation - Hot Shot Method
  • Genotyping
    • Rb
    • Rb conditional
    • Rb1lox recombined
    • p53
    • p53 (Berns/ conditional allele)
    • p53 (Berns/ conditional allele recombined)
    • p53 LSL (reactivatable allele)
    • p53 LSL R172H and p53 LSL R270H (conditional) point mutants
    • p53 R172H and R270H recombined point mutant alleles (germline)
    • p53 Ser23Ala point mutant allele
    • p21
    • Nf1
    • Nf2
    • p107
    • p130
    • p63
    • p73
    • K-ras LA1/2 PCR
    • K-ras LA1/2 Southern
    • K-ras KO
    • K-ras LSL G12D and 1lox recombined
    • H-ras LSL G12D
    • N-ras LSL G12D
    • RosaCreERT2
    • APC flox (exon 14 - Marco Giovaninni's)
    • APC flox (exon 1-14)
    • miR-17~92
    • miR-106a~363 (chromosome X)
    • miR-106b~25 (chromosome 5)
  • Making MEFs
  • pSico and pSicoR

  

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